Classification of the category of diabetes is extremely important for clinicians to diagnose and select the correct treatment plan. Glycosylation, oxidation and other post-translational modifications of membrane and transmembrane proteins, as well as impairment in cholesterol homeostasis, can alter lipid density, packing, and interactions of Red blood cells (RBC) plasma membranes in type 1 and type 2 diabetes, thus varying their membrane micropolarity. This can be estimated, at a submicrometric scale, by determining the membrane relative permittivity, which is the factor by which the electric field between the charges is decreased relative to vacuum. Here, we employed a membrane micropolarity sensitive probe to monitor variations in red blood cells of healthy subjects (n=16) and patients affected by type 1 (T1DM, n=10) and type 2 diabetes mellitus (T2DM, n=24) to provide a cost-effective and supplementary indicator for diabetes classification. We find a less polar membrane microenvironment in T2DM patients, and a more polar membrane microenvironment in T1DM patients compared to control healthy patients. The differences in micropolarity are statistically significant among the three groups (p<0.01). The role of serum cholesterol pool in determining these differences was investigated, and other factors potentially altering the response of the probe were considered in view of developing a clinical assay based on RBC membrane micropolarity. These preliminary data pave the way for the development of an innovative assay which could become a tool for diagnosis and progression monitoring of type 1 and type 2 diabetes.

Bianchetti, G., Di Giacinto, F., Pitocco, D., Rizzi, A., Rizzo, G. E., De Leva, F., Flex, A., Di Stasio, E., Ciasca, G., De Spirito, M., Maulucci, G., Red blood cells membrane micropolarity as a novel diagnostic indicator of type 1 and type 2 diabetes, <<ANALYTICA CHIMICA ACTA>>, 2019; 3 (Novembre): 100030-N/A. [doi:10.1016/j.acax.2019.100030] [http://hdl.handle.net/10807/165495]

Red blood cells membrane micropolarity as a novel diagnostic indicator of type 1 and type 2 diabetes

Bianchetti, Giada
Primo
;
Di Giacinto, Flavio;Pitocco, Dario;Rizzi, Alessandro;De Leva, Francesca;Flex, Andrea;di Stasio, Enrico;Ciasca, Gabriele;De Spirito, Marco
Penultimo
;
Maulucci, Giuseppe
Ultimo
2019

Abstract

Classification of the category of diabetes is extremely important for clinicians to diagnose and select the correct treatment plan. Glycosylation, oxidation and other post-translational modifications of membrane and transmembrane proteins, as well as impairment in cholesterol homeostasis, can alter lipid density, packing, and interactions of Red blood cells (RBC) plasma membranes in type 1 and type 2 diabetes, thus varying their membrane micropolarity. This can be estimated, at a submicrometric scale, by determining the membrane relative permittivity, which is the factor by which the electric field between the charges is decreased relative to vacuum. Here, we employed a membrane micropolarity sensitive probe to monitor variations in red blood cells of healthy subjects (n=16) and patients affected by type 1 (T1DM, n=10) and type 2 diabetes mellitus (T2DM, n=24) to provide a cost-effective and supplementary indicator for diabetes classification. We find a less polar membrane microenvironment in T2DM patients, and a more polar membrane microenvironment in T1DM patients compared to control healthy patients. The differences in micropolarity are statistically significant among the three groups (p<0.01). The role of serum cholesterol pool in determining these differences was investigated, and other factors potentially altering the response of the probe were considered in view of developing a clinical assay based on RBC membrane micropolarity. These preliminary data pave the way for the development of an innovative assay which could become a tool for diagnosis and progression monitoring of type 1 and type 2 diabetes.
Inglese
Bianchetti, G., Di Giacinto, F., Pitocco, D., Rizzi, A., Rizzo, G. E., De Leva, F., Flex, A., Di Stasio, E., Ciasca, G., De Spirito, M., Maulucci, G., Red blood cells membrane micropolarity as a novel diagnostic indicator of type 1 and type 2 diabetes, <<ANALYTICA CHIMICA ACTA>>, 2019; 3 (Novembre): 100030-N/A. [doi:10.1016/j.acax.2019.100030] [http://hdl.handle.net/10807/165495]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10807/165495
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