A new procedure for the encapsulation of non-diffusible drugs into human erythrocytes was developed. With as little as 50 ml of blood and by using a new apparatus, it was possible to encapsulate a variety of biologically active compounds into erythrocytes in 2 h at room temperature and under blood-banking conditions, The process, which is based on two sequential hypotonic dilutions of washed red cells followed by concentration with a haemofilter and resealing of red cells, allows a 35-50% cell recovery and approx, 30% encapsulation of added drugs. The resulting processed erythrocytes have a normal survival in vivo and can be modified further, with the same apparatus, to; Increase their recognition by tissue macrophages to perform as a drug-targeting system. The new equipment designed and built for this procedure was named 'Red Cell Loader'.

Magnani, M., Rossi, L., D'Ascenzo, M., Panzani, I., Bigi, L., Zanella, A., Erythrocyte engineering for drug delivery and targeting, <<BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY>>, 1998; 28 (1): 1-6 [http://hdl.handle.net/10807/161595]

Erythrocyte engineering for drug delivery and targeting

D'Ascenzo, M.
Investigation
;
1998

Abstract

A new procedure for the encapsulation of non-diffusible drugs into human erythrocytes was developed. With as little as 50 ml of blood and by using a new apparatus, it was possible to encapsulate a variety of biologically active compounds into erythrocytes in 2 h at room temperature and under blood-banking conditions, The process, which is based on two sequential hypotonic dilutions of washed red cells followed by concentration with a haemofilter and resealing of red cells, allows a 35-50% cell recovery and approx, 30% encapsulation of added drugs. The resulting processed erythrocytes have a normal survival in vivo and can be modified further, with the same apparatus, to; Increase their recognition by tissue macrophages to perform as a drug-targeting system. The new equipment designed and built for this procedure was named 'Red Cell Loader'.
Inglese
Magnani, M., Rossi, L., D'Ascenzo, M., Panzani, I., Bigi, L., Zanella, A., Erythrocyte engineering for drug delivery and targeting, <<BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY>>, 1998; 28 (1): 1-6 [http://hdl.handle.net/10807/161595]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/161595
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