A new method of orotracheal intubation in mice is described. After intraperitoneal induction of anaesthesia, 36 male animals, belonging to common laboratory strains, have been intubated with the aid of a straight, small bore arthroscope, connected to a video-camera. After the insertion of a guide wire of appropriate size across the vocal cords, a polyethylene (PE) cannula has been introduced over it as an endotracheal tube. Success rate has been 100% both in first intubations and in re-intubations; all procedures have been performed in a mean time of about 3 min. Post-mortem examination of mice did not show any significant damage to upper airway mucosae related to the technique.
Vergari, A., Gunnella, B., Rodola', F., Frassanito, L., Musumeci, M., Palazzesi, S., Casalinuovo, I., A new method of orotracheal intubation in mice, <<EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES>>, 2004; (3): 103-106 [http://hdl.handle.net/10807/16018]
A new method of orotracheal intubation in mice
Vergari, Alessandro;Rodola', Ferdinando;Frassanito, Luciano;
2004
Abstract
A new method of orotracheal intubation in mice is described. After intraperitoneal induction of anaesthesia, 36 male animals, belonging to common laboratory strains, have been intubated with the aid of a straight, small bore arthroscope, connected to a video-camera. After the insertion of a guide wire of appropriate size across the vocal cords, a polyethylene (PE) cannula has been introduced over it as an endotracheal tube. Success rate has been 100% both in first intubations and in re-intubations; all procedures have been performed in a mean time of about 3 min. Post-mortem examination of mice did not show any significant damage to upper airway mucosae related to the technique.
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