MicroRNAs (miRNAs) are intensely studied as candidates for diagnostic and prognostic clinical biomarkers. Faradic impedance spectroscopy (EIS) and differential pulse voltammetry (DPV), coupled to disposable gold electrodes and enzyme amplification of the hybridization event, were used for the development of biosensors for the detection of miRNAs. Biotin-labeled liposomes were employed as nanointerfaces that amplify the primary miRNA-sensing events by their association to the probe–/DNA–miRNA–analyte complex generated onto the transducer.
Voccia, D., Bettazzi, F., Palchetti, I., Electrochemical Liposome-Based Biosensors for Nucleic Acid Detection, in Sensors and Microsystems, (BRESCIA -- ITA, 05-07 February 2013), Springer, Stati Uniti 2014:<<LECTURE NOTES IN ELECTRICAL ENGINEERING>>,268 179-182. [10.1007/978-3-319-00684-0_34] [http://hdl.handle.net/10807/155921]
Electrochemical Liposome-Based Biosensors for Nucleic Acid Detection
Voccia, Diego;
2014
Abstract
MicroRNAs (miRNAs) are intensely studied as candidates for diagnostic and prognostic clinical biomarkers. Faradic impedance spectroscopy (EIS) and differential pulse voltammetry (DPV), coupled to disposable gold electrodes and enzyme amplification of the hybridization event, were used for the development of biosensors for the detection of miRNAs. Biotin-labeled liposomes were employed as nanointerfaces that amplify the primary miRNA-sensing events by their association to the probe–/DNA–miRNA–analyte complex generated onto the transducer.
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