Preliminary results are reported on label-free miRNA determination with faradaic electrochemical impedance spectroscopy (EIS) measurements on glassy carbon electrodes (GCEs) and screen-printed carbon electrodes (SPCEs). These electrodes were coated with films of functionalized conducting polymers. For that, bis(2,2′-bithien-5-yl)-(4-hydroxyphenyl)methane biotin ester was used as the functional monomer. This monomer was potentiodynamically electropolymerized to result in deposition of thin polymer films on the electrodes. Next, streptavidin, and then the biotinylated DNA capture probe were immobilized via streptavidin–biotin interactions. Detectability of the resulting biosensor with respect to the complementary miRNA was well below 100 pM.
Voccia, D., Sosnowska, M., Bettazzi, F., Palchetti, I., Kutner, W., Label-free impedimetric determination of miRNA using biotinylated conducting polymer modified carbon electrodes, in Lecture Notes in Electrical Engineering, (ita, 19-21 February 2014), Springer Verlag, 233 SPRING STREET, NEW YORK, NY 10013, UNITED STATES 2015:<<LECTURE NOTES IN ELECTRICAL ENGINEERING>>,319 59-64. [10.1007/978-3-319-09617-9_11] [http://hdl.handle.net/10807/155920]
Label-free impedimetric determination of miRNA using biotinylated conducting polymer modified carbon electrodes
Voccia, Diego;
2015
Abstract
Preliminary results are reported on label-free miRNA determination with faradaic electrochemical impedance spectroscopy (EIS) measurements on glassy carbon electrodes (GCEs) and screen-printed carbon electrodes (SPCEs). These electrodes were coated with films of functionalized conducting polymers. For that, bis(2,2′-bithien-5-yl)-(4-hydroxyphenyl)methane biotin ester was used as the functional monomer. This monomer was potentiodynamically electropolymerized to result in deposition of thin polymer films on the electrodes. Next, streptavidin, and then the biotinylated DNA capture probe were immobilized via streptavidin–biotin interactions. Detectability of the resulting biosensor with respect to the complementary miRNA was well below 100 pM.
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