Herein, direct determination of small RNAs is described using a functional-polymer modified genosensor. The analytical strategy adopted involves deposition by electropolymerization of biotinylated polythiophene films on the surface of miniaturized, disposable, gold screen-printed electrodes, followed by the layer-by-layer deposition of streptavidin, and then biotynilated capture probes. A small RNA (miR-221) target was determined via the impedimetric measurement of the hybridization event in a label-free and PCR-free approach. Under optimized conditions, the limit of detection (LOD) was 0.7 pM miR-221 (15% RSD). The genosensor was applied for determination of miR-221 in total RNA extracted from human lung and breast cancer cell lines, discriminating between the cancer-positive and -negative cells, without any amplification step, in less than 2 h.

Voccia, D., Sosnowska, M., Bettazzi, F., Roscigno, G., Fratini, E., De Franciscis, V., Condorelli, G., Chitta, R., D'Souza, F., Kutner, W., Palchetti, I., Direct determination of small RNAs using a biotinylated polythiophene impedimetric genosensor, <<BIOSENSORS & BIOELECTRONICS>>, 2016; 87 (N/A): 1012-1019. [doi:10.1016/j.bios.2016.09.058] [http://hdl.handle.net/10807/155914]

Direct determination of small RNAs using a biotinylated polythiophene impedimetric genosensor

Voccia, Diego;
2017

Abstract

Herein, direct determination of small RNAs is described using a functional-polymer modified genosensor. The analytical strategy adopted involves deposition by electropolymerization of biotinylated polythiophene films on the surface of miniaturized, disposable, gold screen-printed electrodes, followed by the layer-by-layer deposition of streptavidin, and then biotynilated capture probes. A small RNA (miR-221) target was determined via the impedimetric measurement of the hybridization event in a label-free and PCR-free approach. Under optimized conditions, the limit of detection (LOD) was 0.7 pM miR-221 (15% RSD). The genosensor was applied for determination of miR-221 in total RNA extracted from human lung and breast cancer cell lines, discriminating between the cancer-positive and -negative cells, without any amplification step, in less than 2 h.
2017
Inglese
Voccia, D., Sosnowska, M., Bettazzi, F., Roscigno, G., Fratini, E., De Franciscis, V., Condorelli, G., Chitta, R., D'Souza, F., Kutner, W., Palchetti, I., Direct determination of small RNAs using a biotinylated polythiophene impedimetric genosensor, <<BIOSENSORS & BIOELECTRONICS>>, 2016; 87 (N/A): 1012-1019. [doi:10.1016/j.bios.2016.09.058] [http://hdl.handle.net/10807/155914]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/155914
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