In this work, we have exploited the unique properties of a chimeric archaeal-human ferritin to encapsulate, deliver and release cytochrome c and induce apoptosis in a myeloid leukemia cell line. The chimeric protein combines the versatility in 24-meric assembly and cargo incorporation capability of Archaeglobus fulgidus ferritin with specific binding of human H ferritin to CD71, the “heavy duty” carrier responsible for transferrin-iron uptake. Delivery of ferritin-encapsulated cytochrome C to the Acute Promyelocytic Leukemia (APL) NB4 cell line, highly resistant to transfection by conventional methods, was successfully achieved in vitro. The effective liberation of cytochrome C within the cytosolic environment, demonstrated by double fluorescent labelling, induced apoptosis in the cancer cells.

Macone, A., Masciarelli, S., Palombarini, F., Quaglio, D., Boffi, A., Trabuco, M. C., Baiocco, P., Fazi, F., Bonamore, A., Ferritin nanovehicle for targeted delivery of cytochrome C to cancer cells, <<SCIENTIFIC REPORTS>>, 2019; 9 (1): 11749-N/A. [doi:10.1038/s41598-019-48037-z] [http://hdl.handle.net/10807/147482]

Ferritin nanovehicle for targeted delivery of cytochrome C to cancer cells

Masciarelli, S.;
2019

Abstract

In this work, we have exploited the unique properties of a chimeric archaeal-human ferritin to encapsulate, deliver and release cytochrome c and induce apoptosis in a myeloid leukemia cell line. The chimeric protein combines the versatility in 24-meric assembly and cargo incorporation capability of Archaeglobus fulgidus ferritin with specific binding of human H ferritin to CD71, the “heavy duty” carrier responsible for transferrin-iron uptake. Delivery of ferritin-encapsulated cytochrome C to the Acute Promyelocytic Leukemia (APL) NB4 cell line, highly resistant to transfection by conventional methods, was successfully achieved in vitro. The effective liberation of cytochrome C within the cytosolic environment, demonstrated by double fluorescent labelling, induced apoptosis in the cancer cells.
2019
Inglese
Macone, A., Masciarelli, S., Palombarini, F., Quaglio, D., Boffi, A., Trabuco, M. C., Baiocco, P., Fazi, F., Bonamore, A., Ferritin nanovehicle for targeted delivery of cytochrome C to cancer cells, <<SCIENTIFIC REPORTS>>, 2019; 9 (1): 11749-N/A. [doi:10.1038/s41598-019-48037-z] [http://hdl.handle.net/10807/147482]
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