We directly tested 484 organisms from clinical (n = 310) and simulated (n = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing Enterobacterales detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives. Among the clinical Klebsiella pneumoniae organisms tested, 122 of 123 KPC, 1 of 1 OXA-48-like, and 1 of 2 VIM producers were detected by the assay. Some VIM and NDM producers yielded scant but still-readable bands with the assay. No organisms produced the IMPS that the assay was designed to detect.

Giordano, L., Fiori, B., D'inzeo, T., Parisi, G., Liotti, F., Menchinelli, G., De Angelis, G., De Maio, F., Luzzaro, F., Sanguinetti, M., Posteraro, B., Spanu, T., Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay, <<ANTIMICROBIAL AGENTS AND CHEMOTHERAPY>>, 2019; 63 (7): N/A-N/A. [doi:10.1128/AAC.00550-19] [http://hdl.handle.net/10807/139382]

Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay

Giordano L
Primo
;
Fiori B;D'Inzeo T;Liotti FM;Menchinelli G;De Angelis G;De Maio F;Sanguinetti M
;
Posteraro B
Penultimo
;
Spanu T
Ultimo
2019

Abstract

We directly tested 484 organisms from clinical (n = 310) and simulated (n = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing Enterobacterales detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives. Among the clinical Klebsiella pneumoniae organisms tested, 122 of 123 KPC, 1 of 1 OXA-48-like, and 1 of 2 VIM producers were detected by the assay. Some VIM and NDM producers yielded scant but still-readable bands with the assay. No organisms produced the IMPS that the assay was designed to detect.
Inglese
https://aac.asm.org/content/aac/63/7/e00550-19.full.pdf
Giordano, L., Fiori, B., D'inzeo, T., Parisi, G., Liotti, F., Menchinelli, G., De Angelis, G., De Maio, F., Luzzaro, F., Sanguinetti, M., Posteraro, B., Spanu, T., Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay, <>, 2019; 63 (7): N/A-N/A. [doi:10.1128/AAC.00550-19] [http://hdl.handle.net/10807/139382]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10807/139382
Citazioni
  • ???jsp.display-item.citation.pmc??? 7
  • Scopus 16
  • ???jsp.display-item.citation.isi??? 17
social impact