We directly tested 484 organisms from clinical (n = 310) and simulated (n = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing Enterobacterales detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives. Among the clinical Klebsiella pneumoniae organisms tested, 122 of 123 KPC, 1 of 1 OXA-48-like, and 1 of 2 VIM producers were detected by the assay. Some VIM and NDM producers yielded scant but still-readable bands with the assay. No organisms produced the IMPS that the assay was designed to detect.
Giordano, L., Fiori, B., D'Inzeo, T., Parisi, G., Liotti, F. M., Menchinelli, G., De Angelis, G., De Maio, F., Luzzaro, F., Sanguinetti, M., Posteraro, B., Spanu, T., Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay, <<ANTIMICROBIAL AGENTS AND CHEMOTHERAPY>>, 2019; 63 (7): N/A-N/A. [doi:10.1128/AAC.00550-19] [http://hdl.handle.net/10807/139382]
Simplified Testing Method for Direct Detection of Carbapenemase-Producing Organisms from Positive Blood Cultures Using the NG-Test Carba 5 Assay
Giordano, LilianaPrimo
;Fiori, Barbara;D'Inzeo, Tiziana;Liotti, Flora Marzia;Menchinelli, Giulia;De Angelis, Giulia;De Maio, Flavio;Sanguinetti, Maurizio
;Posteraro, BrunellaPenultimo
;Spanu, TeresaUltimo
2019
Abstract
We directly tested 484 organisms from clinical (n = 310) and simulated (n = 174) positive blood cultures using the NG-Test Carba 5 assay for carbapenemase-producing Enterobacterales detection. The assay identified all but 4 of the KPC (170/171), OXA-48-like (22/22), VIM (19/21), and NDM (14/15) producers with no false positives. Among the clinical Klebsiella pneumoniae organisms tested, 122 of 123 KPC, 1 of 1 OXA-48-like, and 1 of 2 VIM producers were detected by the assay. Some VIM and NDM producers yielded scant but still-readable bands with the assay. No organisms produced the IMPS that the assay was designed to detect.
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