We evaluated the usefulness of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for Cryptococcus identification at the species and subspecies levels by using an in-house database of 25 reference cryptococcal spectra. Eighty-one out of the 82 Cryptococcus isolates (72 Cryptococcus neoformans and 10 Cryptococcus gattii) tested were correctly identified with respect to their molecular type designations. We showed that MALDI-TOF MS is a practicable alternative to conventional mycology or DNA-based methods.
Posteraro, B., Vella, A., Cogliati, M., De Carolis, E., Florio, A., Posteraro, P., Sanguinetti, M., Tortorano, A., Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii, <<JOURNAL OF CLINICAL MICROBIOLOGY>>, 2012; 50 (7): 2472-2476. [doi:10.1128/JCM.00737-12] [http://hdl.handle.net/10807/13092]
Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry-Based Method for Discrimination between Molecular Types of Cryptococcus neoformans and Cryptococcus gattii
Posteraro, Brunella;Vella, Antonietta;De Carolis, Elena;Sanguinetti, Maurizio;
2012
Abstract
We evaluated the usefulness of matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) for Cryptococcus identification at the species and subspecies levels by using an in-house database of 25 reference cryptococcal spectra. Eighty-one out of the 82 Cryptococcus isolates (72 Cryptococcus neoformans and 10 Cryptococcus gattii) tested were correctly identified with respect to their molecular type designations. We showed that MALDI-TOF MS is a practicable alternative to conventional mycology or DNA-based methods.I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.