In normal pregnancy, villous cytotrophoblast and syncytiotrophoblast do not express HLA Class I and Class II molecules, while invasive extravillous trophoblast only express class I HLA-C and the atypical class Ib antigens, HLA-G, -E and -F. Inadequate maternal tolerance of invasive trophoblast has been proposed as a possible immunologic trigger of poor trophoblast invasion and subsequent occurrence of pre-eclampsia. This study aimed to investigate possible aberrant expression of class II HLA-DR on placentae and syncytiotrophoblast-derived extracellular vesicles (STEVs), obtained by dual placental perfusion, from pre-eclampsia (n = 23) and normal pregnant (n = 14) women. Here we demonstrate that HLA-DR can be detected in syncytiotrophoblast from a significant proportion of pre-eclampsia but not control placentae. HLA-DR was also observed, by flow cytometry, on STEVs and associated with placental alkaline phosphatase to validate their placental origin. HLA-DR positive syncytiotrophoblast was detected in placental biopsies from pre-eclampsia but not normal control cases, using immunohistochemistry. The HLA may be fetal or maternal origin. In the latter case a possible mechanism of acquisition is trogocytosis.

Tersigni, C., Redman, C., Dragovic, R., Tannetta, D., Scambia, G., Di Simone, N., Sargent, I., Vatish, M., HLA-DR is aberrantly expressed at feto-maternal interface in pre-eclampsia, <<JOURNAL OF REPRODUCTIVE IMMUNOLOGY>>, 2018; (Sep;129): 48-52. [doi:10.1016/j.jri.2018.06.024] [http://hdl.handle.net/10807/128687]

HLA-DR is aberrantly expressed at feto-maternal interface in pre-eclampsia

Tersigni, C
Primo
Investigation
;
Scambia, G
Project Administration
;
Di Simone, N
Investigation
;
2018

Abstract

In normal pregnancy, villous cytotrophoblast and syncytiotrophoblast do not express HLA Class I and Class II molecules, while invasive extravillous trophoblast only express class I HLA-C and the atypical class Ib antigens, HLA-G, -E and -F. Inadequate maternal tolerance of invasive trophoblast has been proposed as a possible immunologic trigger of poor trophoblast invasion and subsequent occurrence of pre-eclampsia. This study aimed to investigate possible aberrant expression of class II HLA-DR on placentae and syncytiotrophoblast-derived extracellular vesicles (STEVs), obtained by dual placental perfusion, from pre-eclampsia (n = 23) and normal pregnant (n = 14) women. Here we demonstrate that HLA-DR can be detected in syncytiotrophoblast from a significant proportion of pre-eclampsia but not control placentae. HLA-DR was also observed, by flow cytometry, on STEVs and associated with placental alkaline phosphatase to validate their placental origin. HLA-DR positive syncytiotrophoblast was detected in placental biopsies from pre-eclampsia but not normal control cases, using immunohistochemistry. The HLA may be fetal or maternal origin. In the latter case a possible mechanism of acquisition is trogocytosis.
Inglese
Tersigni, C., Redman, C., Dragovic, R., Tannetta, D., Scambia, G., Di Simone, N., Sargent, I., Vatish, M., HLA-DR is aberrantly expressed at feto-maternal interface in pre-eclampsia, <<JOURNAL OF REPRODUCTIVE IMMUNOLOGY>>, 2018; (Sep;129): 48-52. [doi:10.1016/j.jri.2018.06.024] [http://hdl.handle.net/10807/128687]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/128687
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