A reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for identification and estimation of 18-β-glycyrrhetinic acid (GA) in HepG2 cell line. The analysis was carried out using a JASCO HPLC system with a C-18 (3 μm) Supelco reversed phase column (150 x 4.7 mm) using a mobile phase of 80% CH3OH and 20% of CH3CN: tetrahydrofuran: water (10:80:10, v/v/v). The method was linear in the concentration range of 1.5–120 μg /mL (n = 5). The LOD and LOQ were determined based on standard deviation of the y-intercept and the slope of the calibration curve. The LOD and LOQ values were found to be 11.46 μg/mL and 34.72 μg/mL, respectively. The mean percentage recovery by standard addition experiments of GA is 92.4 % ± 5.2%. The intracellular GA concentration value, obtained as mean of five different determinations, was 45.8 ± 7.45 μg/mL. We have developed a HPLC-UV method for quantitative determination of GA inside cells, with advantages in the cost reduction and economy of the analytical process.

Nocca, G., Calla', C. A. M., Santini, S. A., Amalfitano, A., Marigo, L., Rossetti, D. V., Spagnuolo, G., Cordaro, M., Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method., <<INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY>>, 2018; 2018 (2018): 1-5. [doi:10.1155/2018/5673186] [http://hdl.handle.net/10807/128360]

Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method.

Nocca, Giuseppina
Primo
;
Calla', Cinzia Anna Maria
Secondo
;
Santini, Stefano Angelo;Amalfitano, Adriana;Marigo, Luca;Rossetti, Diana Valeria;Cordaro, Massimo
Ultimo
2018

Abstract

A reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for identification and estimation of 18-β-glycyrrhetinic acid (GA) in HepG2 cell line. The analysis was carried out using a JASCO HPLC system with a C-18 (3 μm) Supelco reversed phase column (150 x 4.7 mm) using a mobile phase of 80% CH3OH and 20% of CH3CN: tetrahydrofuran: water (10:80:10, v/v/v). The method was linear in the concentration range of 1.5–120 μg /mL (n = 5). The LOD and LOQ were determined based on standard deviation of the y-intercept and the slope of the calibration curve. The LOD and LOQ values were found to be 11.46 μg/mL and 34.72 μg/mL, respectively. The mean percentage recovery by standard addition experiments of GA is 92.4 % ± 5.2%. The intracellular GA concentration value, obtained as mean of five different determinations, was 45.8 ± 7.45 μg/mL. We have developed a HPLC-UV method for quantitative determination of GA inside cells, with advantages in the cost reduction and economy of the analytical process.
2018
Inglese
Nocca, G., Calla', C. A. M., Santini, S. A., Amalfitano, A., Marigo, L., Rossetti, D. V., Spagnuolo, G., Cordaro, M., Quantitative Determination of 18-β-Glycyrrhetinic Acid in HepG2 Cell Line by High Performance Liquid Chromatography Method., <<INTERNATIONAL JOURNAL OF ANALYTICAL CHEMISTRY>>, 2018; 2018 (2018): 1-5. [doi:10.1155/2018/5673186] [http://hdl.handle.net/10807/128360]
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