Rescue of embryonic lethality in MDM4-/- mice through concomitant loss of p53 has revealed a functional partnership between the two proteins. Biochemical studies have suggested that MDM4 may act as a negative regulator of p53 levels and activity. On the other hand, MDM4 overexpression has been reported to stabilize p53 levels and to counteract MDM2-degradative activity. We have investigated the functional role of MDM4 overexpression on cell behaviour. In both established and primary cells cultured under stress conditions, overexpression of MDM4 significantly increased p53-dependent cell death, in correlation with enhanced induction of the endogenous p53 protein levels. This phenomenon was associated with induced p53 transcriptional activity and increased levels of the pro-apoptotic protein, Bax. Further, p53 stabilization was accompanied by decreased association of the protein to its negative regulator, MDM2. These findings reveal a novel role for MDM4 by demonstrating that in non-tumor cells under stress conditions it may act as a positive regulator of p53 activity, by mainly controlling p53 levels. They also indicate a major distinction between the biological consequences of MDM4 and MDM2 overexpression

Mancini, F., Gentiletti, F., D'angelo, M., Giglio, S., Nanni, S., D'angelo, C., Farsetti, A., Citro, G., Sacchi, A., Pontecorvi, A., Moretti, F., MDM4 (MDMX) overexpression enhances stabilization of stress-induced p53 and promotes apoptosis., <<THE JOURNAL OF BIOLOGICAL CHEMISTRY>>, 2004; (279 (9)): 8169-8180 [http://hdl.handle.net/10807/12475]

MDM4 (MDMX) overexpression enhances stabilization of stress-induced p53 and promotes apoptosis.

Nanni, Simona;Farsetti, Antonella;Pontecorvi, Alfredo;Moretti, Fabiola
2004

Abstract

Rescue of embryonic lethality in MDM4-/- mice through concomitant loss of p53 has revealed a functional partnership between the two proteins. Biochemical studies have suggested that MDM4 may act as a negative regulator of p53 levels and activity. On the other hand, MDM4 overexpression has been reported to stabilize p53 levels and to counteract MDM2-degradative activity. We have investigated the functional role of MDM4 overexpression on cell behaviour. In both established and primary cells cultured under stress conditions, overexpression of MDM4 significantly increased p53-dependent cell death, in correlation with enhanced induction of the endogenous p53 protein levels. This phenomenon was associated with induced p53 transcriptional activity and increased levels of the pro-apoptotic protein, Bax. Further, p53 stabilization was accompanied by decreased association of the protein to its negative regulator, MDM2. These findings reveal a novel role for MDM4 by demonstrating that in non-tumor cells under stress conditions it may act as a positive regulator of p53 activity, by mainly controlling p53 levels. They also indicate a major distinction between the biological consequences of MDM4 and MDM2 overexpression
Inglese
Mancini, F., Gentiletti, F., D'angelo, M., Giglio, S., Nanni, S., D'angelo, C., Farsetti, A., Citro, G., Sacchi, A., Pontecorvi, A., Moretti, F., MDM4 (MDMX) overexpression enhances stabilization of stress-induced p53 and promotes apoptosis., <<THE JOURNAL OF BIOLOGICAL CHEMISTRY>>, 2004; (279 (9)): 8169-8180 [http://hdl.handle.net/10807/12475]
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/10807/12475
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