The use of pancreatic islet transplantation in membrane bioreactors put in vascular circuits aims at resetting the glucose homeostasis in diabetic or pancreatectomized patients, avoiding immune host rejection. Our experience was carried out at following stages: porcine pancreas explantation and enzymatic separation of endocrine tissue from exocrine fraction by collagenase; evaluation of islet functionality (culture tests); in vitro tests of the islets-bioreactor system, to assess the metabolic response to the glucose; in vivo evaluation to assay the haemodynamic behaviour. The trials showed a good metabolic bioreactor functionality and a decreasing incidence of coagulative problems.
Urso, A., Careddu, G., Ghirlanda, G., Catapano, G., Brisinda, G., Ceriati, F., Bellantone, R. D. A., Doglietto, G., Crucitti, F., Hybrid artificial pancreas: islet transplantation inside membrane bioreactors, <<BIOMATERIALS, ARTIFICIAL CELLS, AND IMMOBILIZATION BIOTECHNOLOGY>>, 1992; 20 (5): 1177-1192 [http://hdl.handle.net/10807/11936]
Hybrid artificial pancreas: islet transplantation inside membrane bioreactors
Urso, A;Catapano, G;Ceriati, Franco;Bellantone, Rocco Domenico Alfonso;Doglietto, Gb;
1992
Abstract
The use of pancreatic islet transplantation in membrane bioreactors put in vascular circuits aims at resetting the glucose homeostasis in diabetic or pancreatectomized patients, avoiding immune host rejection. Our experience was carried out at following stages: porcine pancreas explantation and enzymatic separation of endocrine tissue from exocrine fraction by collagenase; evaluation of islet functionality (culture tests); in vitro tests of the islets-bioreactor system, to assess the metabolic response to the glucose; in vivo evaluation to assay the haemodynamic behaviour. The trials showed a good metabolic bioreactor functionality and a decreasing incidence of coagulative problems.
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