Results of cultured islet transplantation in the management of insulin-dependent diabetes are still unsatisfactory. The main problem preventing success is the swift and resolute host immune rejection. To obviate this we designed and experimented a model of bioartificial pancreas, made of polymeric hollow fibers, put into the blood circulation as an artero-venous bypass to immunoisolate endocrine tissue from leucocytes and immunoglobulins. We tested four different membrane bioreactors (BR1-4). BR1 and 2 had seven hollow fibers, the others more than 6,000 smaller fibers. In BR4 a connecting tube with a high-permeability membrane was inserted between the islet compartment and the bioreactor outlet to improve the ultrafiltration flow. In vitro, the islets inside the bioreactor perfused with glucose solutions (300 mg%) showed a rapid, high insulin secretory response, related to the glucose stimulation. The use of the outside connection allowed a twofold increase of insulin production.

Lombardi, C. P., Urso, A., Catapano, G., Careddu, G., Ghirlanda, G., Ceriati, F., Brisinda, G., Bellantone, R. D. A., Doglietto, G., Crucitti, F., Membrane bioreactors as hybrid artificial pancreas: experimental evaluation, <<INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS>>, 1992; 15 (2): 126-130 [http://hdl.handle.net/10807/11929]

Membrane bioreactors as hybrid artificial pancreas: experimental evaluation

Lombardi, Celestino Pio;Ghirlanda, Giovanni;Ceriati, Franco;Brisinda, Giuseppe;Bellantone, Rocco Domenico Alfonso;
1992

Abstract

Results of cultured islet transplantation in the management of insulin-dependent diabetes are still unsatisfactory. The main problem preventing success is the swift and resolute host immune rejection. To obviate this we designed and experimented a model of bioartificial pancreas, made of polymeric hollow fibers, put into the blood circulation as an artero-venous bypass to immunoisolate endocrine tissue from leucocytes and immunoglobulins. We tested four different membrane bioreactors (BR1-4). BR1 and 2 had seven hollow fibers, the others more than 6,000 smaller fibers. In BR4 a connecting tube with a high-permeability membrane was inserted between the islet compartment and the bioreactor outlet to improve the ultrafiltration flow. In vitro, the islets inside the bioreactor perfused with glucose solutions (300 mg%) showed a rapid, high insulin secretory response, related to the glucose stimulation. The use of the outside connection allowed a twofold increase of insulin production.
1992
Inglese
Lombardi, C. P., Urso, A., Catapano, G., Careddu, G., Ghirlanda, G., Ceriati, F., Brisinda, G., Bellantone, R. D. A., Doglietto, G., Crucitti, F., Membrane bioreactors as hybrid artificial pancreas: experimental evaluation, <<INTERNATIONAL JOURNAL OF ARTIFICIAL ORGANS>>, 1992; 15 (2): 126-130 [http://hdl.handle.net/10807/11929]
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/11929
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact