The Linear Ubiquitin chain Assembly Complex (LUBAC) is required for optimal gene activation and prevention of cell death upon activation of immune receptors, including TNFR1. Deficiency in the LUBAC components SHARPIN or HOIP in mice results in severe inflammation in adulthood or embryonic lethality, respectively, due to deregulation of TNFR1-mediated cell death. In humans, deficiency in the third LUBAC component, HOIL-1, causes autoimmunity and inflammatory disease, similar to HOIP deficiency, whereas HOIL-1 deficiency in mice was reported to cause no overt phenotype. By creating HOIL-1-deficient mice, we here show that HOIL-1 is, however, as essential for LUBAC function as HOIP, albeit for different reasons: whereas HOIP is LUBAC's catalytically active component, HOIL-1 is required for LUBAC assembly, stability and optimal retention in the TNFR1-signalling complex (TNFR1-SC), thereby preventing aberrant cell death. Both, HOIL-1 and HOIP prevent embryonic lethality at mid-gestation by interfering with aberrant TNFR1-mediated endothelial cell death, which only partially depends on RIPK1 kinase activity. Co-deletion of Caspase-8 with RIPK3 or MLKL prevents cell death in Hoil-1-/- embryos, yet only combined loss of Caspase-8 with MLKL results in viable HOIL-1-deficient mice. Interestingly, Ripk3-/-Caspase-8-/-Hoil-1-/- embryos die at late-gestation due to haematopoietic defects that are rescued by co-deletion of RIPK1 but not MLKL. Collectively, these results demonstrate that both, HOIP and HOIL-1 are essential LUBAC components and are required for embryogenesis by preventingaberrant cell death. Furthermore, they unveil that, when LUBAC and Caspase-8 are absent, RIPK3 prevents RIPK1 from inducing embryonic lethality by causing defects in foetal haematopoiesis.

Nieves, P., Maurice, D., Antonella, M., Peter, D., Helena, D., Sebastian, K., Eva, R., Amanda, F., Ciaran, H., Lucia, T., Torsten, H., Elodie, L., Haas, T. L., Yutaka, S., Charlotta, B., Aida, S., James, R., Silvia, A., Michael T., A., Allison, B., Tariq, E., John, B., William, K., Andreas, S., John, S., Philippe Bouillet And Henning, W., LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis, <<NATURE>>, N/A; (N/A): N/A-N/A [http://hdl.handle.net/10807/116933]

LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis

Haas, Tobias Longin;
2018

Abstract

The Linear Ubiquitin chain Assembly Complex (LUBAC) is required for optimal gene activation and prevention of cell death upon activation of immune receptors, including TNFR1. Deficiency in the LUBAC components SHARPIN or HOIP in mice results in severe inflammation in adulthood or embryonic lethality, respectively, due to deregulation of TNFR1-mediated cell death. In humans, deficiency in the third LUBAC component, HOIL-1, causes autoimmunity and inflammatory disease, similar to HOIP deficiency, whereas HOIL-1 deficiency in mice was reported to cause no overt phenotype. By creating HOIL-1-deficient mice, we here show that HOIL-1 is, however, as essential for LUBAC function as HOIP, albeit for different reasons: whereas HOIP is LUBAC's catalytically active component, HOIL-1 is required for LUBAC assembly, stability and optimal retention in the TNFR1-signalling complex (TNFR1-SC), thereby preventing aberrant cell death. Both, HOIL-1 and HOIP prevent embryonic lethality at mid-gestation by interfering with aberrant TNFR1-mediated endothelial cell death, which only partially depends on RIPK1 kinase activity. Co-deletion of Caspase-8 with RIPK3 or MLKL prevents cell death in Hoil-1-/- embryos, yet only combined loss of Caspase-8 with MLKL results in viable HOIL-1-deficient mice. Interestingly, Ripk3-/-Caspase-8-/-Hoil-1-/- embryos die at late-gestation due to haematopoietic defects that are rescued by co-deletion of RIPK1 but not MLKL. Collectively, these results demonstrate that both, HOIP and HOIL-1 are essential LUBAC components and are required for embryogenesis by preventingaberrant cell death. Furthermore, they unveil that, when LUBAC and Caspase-8 are absent, RIPK3 prevents RIPK1 from inducing embryonic lethality by causing defects in foetal haematopoiesis.
Inglese
Nieves, P., Maurice, D., Antonella, M., Peter, D., Helena, D., Sebastian, K., Eva, R., Amanda, F., Ciaran, H., Lucia, T., Torsten, H., Elodie, L., Haas, T. L., Yutaka, S., Charlotta, B., Aida, S., James, R., Silvia, A., Michael T., A., Allison, B., Tariq, E., John, B., William, K., Andreas, S., John, S., Philippe Bouillet And Henning, W., LUBAC is essential for embryogenesis by preventing cell death and enabling haematopoiesis, <<NATURE>>, N/A; (N/A): N/A-N/A [http://hdl.handle.net/10807/116933]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/116933
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