Permanent cell cultures are invaluable tools for understanding the biological characteristics of tumors. In the present study the authors report on the establishment of permanent human cell lines from three cases of aggressive chordomas of the clival region. All of the parental tumors showed telomerase activity. Cultured chordoma cells had a doubling time of 5 to 7 days and grew as a monolayer of cells that retained both the immunophenotype and the p53 status of the parental tumor. In vitro, chordoma cells overexpressed telomerase, supporting the hypothesis that this enzyme is required for the immortalization process.
Ricci-Vitiani, L., Pierconti, F., Falchetti, M. L., Petrucci, G., Maira, G., De Maria Marchiano, R., Larocca, L. M., Pallini, R., Establishing tumor cell lines from aggressive telomerase-positive chordomas of the skull base: Technical note, <<JOURNAL OF NEUROSURGERY>>, 2006; 105 (3): 482-484. [doi:10.3171/jns.2006.105.3.482] [http://hdl.handle.net/10807/113391]
Establishing tumor cell lines from aggressive telomerase-positive chordomas of the skull base: Technical note
Pierconti, Francesco;Petrucci, Giovanna;Maira, Giulio;De Maria Marchiano, Ruggero;Larocca, Luigi Maria;Pallini, Roberto
2006
Abstract
Permanent cell cultures are invaluable tools for understanding the biological characteristics of tumors. In the present study the authors report on the establishment of permanent human cell lines from three cases of aggressive chordomas of the clival region. All of the parental tumors showed telomerase activity. Cultured chordoma cells had a doubling time of 5 to 7 days and grew as a monolayer of cells that retained both the immunophenotype and the p53 status of the parental tumor. In vitro, chordoma cells overexpressed telomerase, supporting the hypothesis that this enzyme is required for the immortalization process.
I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.
