E. faecalis and E. faecium cause urinary tract infections highly resistant to therapies due to a protective extracellular matrix. To exploit a new strategy able to treat infections without increasing antibiotic doses, we used enzymes targeting specific biofilm matrix components in combination with Vancomycin. We investigated the activity of Vancomycin combined with two matrix-degrading enzymes, Alginate Lyase (AlgL) and Deoxyribonuclease I (DNase I) against in vitro biofilm of E. faecalis and E. faecium clinical isolates. The heterogeneity of matrix composition leads to defined physiological responses of biofilm communities to their environment: we demonstrated that the use of DNase I and AlgL enzymes affects biofilm structure, cell viability and reduces MBEC values of Vancomycin in E. faecalis and E. faecium, respectively.

Torelli, R., Cacaci, M., Papi, M., Paroni Sterbini, F., Martini, C., Posteraro, B., Palmieri, V., De Spirito, M., Sanguinetti, M., Bugli, F., Different effects of matrix degrading enzymes towards biofilms formed by E. faecalis and E. faecium clinical isolates, <<COLLOIDS AND SURFACES. B, BIOINTERFACES>>, 2017; 158 (ottobre): 349-355. [doi:10.1016/j.colsurfb.2017.07.010] [http://hdl.handle.net/10807/109765]

Different effects of matrix degrading enzymes towards biofilms formed by E. faecalis and E. faecium clinical isolates

Torelli, Riccardo
Primo
;
Cacaci, Margherita;Papi, Massimiliano
;
Paroni Sterbini, Francesco;Martini, Cecilia;Posteraro, Brunella;Palmieri, Valentina;De Spirito, Marco;Sanguinetti, Maurizio;Bugli, Francesca
Ultimo
2017

Abstract

E. faecalis and E. faecium cause urinary tract infections highly resistant to therapies due to a protective extracellular matrix. To exploit a new strategy able to treat infections without increasing antibiotic doses, we used enzymes targeting specific biofilm matrix components in combination with Vancomycin. We investigated the activity of Vancomycin combined with two matrix-degrading enzymes, Alginate Lyase (AlgL) and Deoxyribonuclease I (DNase I) against in vitro biofilm of E. faecalis and E. faecium clinical isolates. The heterogeneity of matrix composition leads to defined physiological responses of biofilm communities to their environment: we demonstrated that the use of DNase I and AlgL enzymes affects biofilm structure, cell viability and reduces MBEC values of Vancomycin in E. faecalis and E. faecium, respectively.
2017
Inglese
Torelli, R., Cacaci, M., Papi, M., Paroni Sterbini, F., Martini, C., Posteraro, B., Palmieri, V., De Spirito, M., Sanguinetti, M., Bugli, F., Different effects of matrix degrading enzymes towards biofilms formed by E. faecalis and E. faecium clinical isolates, <<COLLOIDS AND SURFACES. B, BIOINTERFACES>>, 2017; 158 (ottobre): 349-355. [doi:10.1016/j.colsurfb.2017.07.010] [http://hdl.handle.net/10807/109765]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/109765
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