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EnglishBackground: Anti-D monoclonal antibody (MoAb) reagents have improved D typing in routine tests. However, they exhibit a wide range of reactivity with the weak D phenotype depending on the characteristics of the different MoAbs used. We analyzed the reactivity of immunoglobulin (IgM) anti-D by cluster analysis to characterize MoAb that have similar reactivities with the weak D phenotype. Study Design and Methods: We used 36 consecutive samples with weak D phenotype in routine testing and determined their reactivity with different IgM and IgG anti-D MoAbs. The samples were characterized as belonging to a weak D type or category using commercial molecular biology kits. Results: The various anti-D MoAbs showed a wide grade of reactivity with the weak D samples. Similarities and dissimilarities in the behavior of the anti-D MoAbs with the weak D phenotype samples were detected with cluster analysis and the multidimensional scaling analysis. These analyses indicated different families of MoAbs characterized as having a high degree of homogeneity in their reactivity with the weak D phenotype. Between these MoAb families, the most effective at reacting with the weak D phenotype were RUM-1 and 175-2. CONCLUSIONS: The results show that it is possible to classify the anti-D MoAbs on the basis of their reactivity with the weak D phenotype. This provides information about different MoAbs' properties on the basis of their belonging to a given of anti-D family. © 2008 American Association of Blood Banks.
Pascali, V. L., Lai, M., Grasso, C., Boschi, I., D'onofrio, G., Leone, G., Characterization of anti-D monoclonal antibody reagents based on their reactivity with the weak D phenotype, <<TRANSFUSION>>, 2009; (Maggio): 937-942 [http://hdl.handle.net/10807/10849]
| Autori: | ||
| Titolo: | Characterization of anti-D monoclonal antibody reagents based on their reactivity with the weak D phenotype | |
| Data di pubblicazione: | 2009 | |
| Abstract: | Background: Anti-D monoclonal antibody (MoAb) reagents have improved D typing in routine tests. However, they exhibit a wide range of reactivity with the weak D phenotype depending on the characteristics of the different MoAbs used. We analyzed the reactivity of immunoglobulin (IgM) anti-D by cluster analysis to characterize MoAb that have similar reactivities with the weak D phenotype. Study Design and Methods: We used 36 consecutive samples with weak D phenotype in routine testing and determined their reactivity with different IgM and IgG anti-D MoAbs. The samples were characterized as belonging to a weak D type or category using commercial molecular biology kits. Results: The various anti-D MoAbs showed a wide grade of reactivity with the weak D samples. Similarities and dissimilarities in the behavior of the anti-D MoAbs with the weak D phenotype samples were detected with cluster analysis and the multidimensional scaling analysis. These analyses indicated different families of MoAbs characterized as having a high degree of homogeneity in their reactivity with the weak D phenotype. Between these MoAb families, the most effective at reacting with the weak D phenotype were RUM-1 and 175-2. CONCLUSIONS: The results show that it is possible to classify the anti-D MoAbs on the basis of their reactivity with the weak D phenotype. This provides information about different MoAbs' properties on the basis of their belonging to a given of anti-D family. © 2008 American Association of Blood Banks. | |
| Lingua: | Inglese | |
| Rivista: | ||
| Citazione: | Pascali, V. L., Lai, M., Grasso, C., Boschi, I., D'onofrio, G., Leone, G., Characterization of anti-D monoclonal antibody reagents based on their reactivity with the weak D phenotype, <<TRANSFUSION>>, 2009; (Maggio): 937-942 [http://hdl.handle.net/10807/10849] | |
| Appare nelle tipologie: | Articolo in rivista, Nota a sentenza |
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