Several studies have reported that dental resin-based materials release substances with biological liability. In this work the in vitro cytotoxic potential of specimens of a self-curing and a light-curing orthodontic composite resins - prepared according to the producer instructions - was evaluated using mouse fibroblast cell line (3T3 Swiss) with the following cell-material contact systems: 1. 24 h extracts toxicity. The extracts of specimens, left for 24 h in cell culture medium, were then placed in contact with 3T3 fibroblasts for 24h; the cytotoxicity was evaluated using the thiazolyl blue tetrazolium bromide (MTT) assay. 2. Indirect method. The specimens were settled - for a period of 24 h - on a tissue culture insert placed over the cellular monolayer; the cytotoxicity of the resin components released was then tested by MTT. The results, obtained in this study, elicit a close agreement between the two procedures; from the data obtained in the reported experimental conditions, it is possible to establish that the examined chemical-cured material was more cytotoxic than the light-cured one. Further investigations about the influence of the modes of polymerization of the materials on the biological effects are warranted to verify their clinical implications.

Nocca, G., Chimenti, C., Parziale, V., Gambarini, G., Giardina, B., Lupi, A., In vitro comparison of the cytotoxicity of two orthodontic composite resins., <<MINERVA STOMATOLOGICA>>, 2006; (55): 297-305 [http://hdl.handle.net/10807/10446]

In vitro comparison of the cytotoxicity of two orthodontic composite resins.

Nocca, Giuseppina;Giardina, Bruno;Lupi, Alessandro
2006

Abstract

Several studies have reported that dental resin-based materials release substances with biological liability. In this work the in vitro cytotoxic potential of specimens of a self-curing and a light-curing orthodontic composite resins - prepared according to the producer instructions - was evaluated using mouse fibroblast cell line (3T3 Swiss) with the following cell-material contact systems: 1. 24 h extracts toxicity. The extracts of specimens, left for 24 h in cell culture medium, were then placed in contact with 3T3 fibroblasts for 24h; the cytotoxicity was evaluated using the thiazolyl blue tetrazolium bromide (MTT) assay. 2. Indirect method. The specimens were settled - for a period of 24 h - on a tissue culture insert placed over the cellular monolayer; the cytotoxicity of the resin components released was then tested by MTT. The results, obtained in this study, elicit a close agreement between the two procedures; from the data obtained in the reported experimental conditions, it is possible to establish that the examined chemical-cured material was more cytotoxic than the light-cured one. Further investigations about the influence of the modes of polymerization of the materials on the biological effects are warranted to verify their clinical implications.
Inglese
Nocca, G., Chimenti, C., Parziale, V., Gambarini, G., Giardina, B., Lupi, A., In vitro comparison of the cytotoxicity of two orthodontic composite resins., <<MINERVA STOMATOLOGICA>>, 2006; (55): 297-305 [http://hdl.handle.net/10807/10446]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/10446
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