Banks of genetically characterized cancer stem cells (CSCs) isolated from individual patients and grown as spheroids offer an invaluable approach to identify genetic determinants of drug resistance versus sensitivity, and to study new stem cell-directed therapies. Here, we describe our standardized procedure for in vitro drug screening on colorectal CSCs, taking irinotecan as an example.

De Angelis, M. L., De Maria Marchiano, R., Baiocchi, M., How to assess drug resistance in cancer stem cells, in Gianpaolo Papaccio And Vincenzo Desideri, G. P. A. V. D. (ed.), Cancer Stem Cells: Methods and Protocols, Methods in Molecular Biology,, HUMANA PRESS INC, 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA 2018: <<METHODS IN MOLECULAR BIOLOGY>>, 1692 107- 115. 10.1007/978-1-4939-7401-6_10 [http://hdl.handle.net/10807/111830]

How to assess drug resistance in cancer stem cells

De Angelis, Maria Laura;De Maria Marchiano, Ruggero;
2018

Abstract

Banks of genetically characterized cancer stem cells (CSCs) isolated from individual patients and grown as spheroids offer an invaluable approach to identify genetic determinants of drug resistance versus sensitivity, and to study new stem cell-directed therapies. Here, we describe our standardized procedure for in vitro drug screening on colorectal CSCs, taking irinotecan as an example.
2018
Inglese
Cancer Stem Cells: Methods and Protocols, Methods in Molecular Biology,
9781493974009
HUMANA PRESS INC
1692
De Angelis, M. L., De Maria Marchiano, R., Baiocchi, M., How to assess drug resistance in cancer stem cells, in Gianpaolo Papaccio And Vincenzo Desideri, G. P. A. V. D. (ed.), Cancer Stem Cells: Methods and Protocols, Methods in Molecular Biology,, HUMANA PRESS INC, 999 RIVERVIEW DR, STE 208, TOTOWA, NJ 07512-1165 USA 2018: <<METHODS IN MOLECULAR BIOLOGY>>, 1692 107- 115. 10.1007/978-1-4939-7401-6_10 [http://hdl.handle.net/10807/111830]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/111830
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