Banks of genetically characterized cancer stem cells (CSCs) isolated from individual patients and grown as spheroids offer an invaluable approach to identify genetic determinants of drug resistance versus sensitivity, and to study new stem cell-directed therapies. Here, we describe our standardized procedure for in vitro drug screening on colorectal CSCs, taking irinotecan as an example.
De Angelis, M. L., De Maria Marchiano, R., Marta, B., How to Assess Drug Resistance in Cancer Stem Cells, in Gianpaolo Papaccio And Vincenzo Desideri, G. P. A. V. D. (ed.), Cancer Stem Cells: Methods and Protocols, Springer Science & Business Media, LONDON 2017: <<METHODS IN MOLECULAR BIOLOGY>>, N/A- N/A. 10.1007/978-1-4939-7401-6_10 [http://hdl.handle.net/10807/111255]
How to Assess Drug Resistance in Cancer Stem Cells
De Angelis, Maria Laura;De Maria Marchiano, Ruggero;
2017
Abstract
Banks of genetically characterized cancer stem cells (CSCs) isolated from individual patients and grown as spheroids offer an invaluable approach to identify genetic determinants of drug resistance versus sensitivity, and to study new stem cell-directed therapies. Here, we describe our standardized procedure for in vitro drug screening on colorectal CSCs, taking irinotecan as an example.
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