The distribution of haemoglobin components in erythrocytes has been determined by single-cell spectrophotometry. In the case of trout blood, which contains three major haemoglobin components, it has been shown that all erythrocytes contain the component characterized by the Root effect (Hb trout IV) in a proportion very similar to that found in the haemolysate. The method described here also appears to have great possibilities in the study of the distribution, among and within the erythrocytes, of abnormal human haemoglobin.
Brunori, M., Giardina, B., Antonini, E., B. E. N. E. D. E. T. T. Pa, N., B. I. A. N. C. H. I. N. G, N., DISTRIBUTION OF HEMOGLOBIN COMPONENTS OF TROUT BLOOD AMONG ERYTHROCYTES - OBSERVATIONS BY SINGLE-CELL SPECTROSCOPY, <<JOURNAL OF MOLECULAR BIOLOGY>>, 1974; 86 (1): 165-169. [doi:10.1016/S0022-2836(74)80015-X] [http://hdl.handle.net/10807/6036]
DISTRIBUTION OF HEMOGLOBIN COMPONENTS OF TROUT BLOOD AMONG ERYTHROCYTES - OBSERVATIONS BY SINGLE-CELL SPECTROSCOPY
Giardina, Bruno;
1974
Abstract
The distribution of haemoglobin components in erythrocytes has been determined by single-cell spectrophotometry. In the case of trout blood, which contains three major haemoglobin components, it has been shown that all erythrocytes contain the component characterized by the Root effect (Hb trout IV) in a proportion very similar to that found in the haemolysate. The method described here also appears to have great possibilities in the study of the distribution, among and within the erythrocytes, of abnormal human haemoglobin.
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