Effects of dental methacrylates on oxygen consumption and redox status of human pulp cells

Several studies have already demonstrated that the incomplete polymerization of resin based dental materials causes the release of monomers which might affect cell metabolism. The aim of this study was to investigate the effects of triethylenglycol-dimethacrylate, 1,4-butanediol-dimethacrylate, urethane-dimethacrylate and 2-hydroxyethyl-methacrylate on 1) cellular energy metabolism, evaluating oxygen consumption rate, glucose consumption, glucose 6-phosphate dehydrogenase activity, and lactate production 2) cellular redox status, through the evaluation of glutathione concentration and of the activities of enzymes regulating glutathione metabolism. Methods: Human pulp cells were used and oxygen consumption was measured by means of a Clark electrode. Moreover, reactive oxygen species production was quantified. Enzymatic activity, glucose and lactate concentrations were determined through a specific kit. Results: triethylenglycol-dimethacrylate, 1,4-butanediol-dimethacrylate and 2-hydroxyethyl-methacrylate induced a decrease in oxygen consumption rate, an enhancement of glucose consumption and lactate production, whilst glucose 6-phosphate dehydrogenase and glutathione reductase activity were not significantly modified. Moreover, the monomers induced an increase of reactive oxygen species production with a consequent increase of superoxide dismutase and catalase enzymatic activities. A depletion of both reduced and total glutathione was also observed. Conclusion: The obtained results indicate that dental monomers might alter energy 44 metabolism and glutathione redox balance in human pulp cells