In recent years various kinds of biosensors for the detection of pathogens have been developed. A genosensor consists in the immobilization, onto the surface of a chosen transducer, of an oligonucleotide with a specific base sequence called capture probe. The complementary sequence (the analytical target, i.e. a specific sequence of the DNA/RNA of the pathogen) present in the sample is recognized and captured by the probe through the hybridization reaction. The evaluation of the extent of the hybridization allows one to confirm whether the sample contains the complementary sequence of the probe or not. Electrochemical transducers have received considerable attention in connection with the detection of DNA hybridization. Moreover, recently, with the emergence of novel photoelectrochemically active species and new detection schemes, photoelectrochemistry has resulted in substantial progress in its analytical performance for biosensing applications. In this paper, some examples of electrochemical genosensors for multiplexed pathogen detection are shown. Moreover, the preliminary experiments towards the development of a photoelectrochemical genosensor using a TiO2-nanocrystal-modified ITO electrode are discussed.

Voccia, D., Bettazi, F., Palchetti, I., Different strategies for the detection of bioagents using electrochemical and photoelectrochemical genosensors, Abstract de <<Optics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII>>, (fra, 21-25 September 2015 ), SPIE, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA 2015:<<PROCEEDINGS OF SPIE, THE INTERNATIONAL SOCIETY FOR OPTICAL ENGINEERING>>,9652 96520T-96520T. 10.1117/12.2194838 [http://hdl.handle.net/10807/155917]

Different strategies for the detection of bioagents using electrochemical and photoelectrochemical genosensors

Voccia, Diego;
2015

Abstract

In recent years various kinds of biosensors for the detection of pathogens have been developed. A genosensor consists in the immobilization, onto the surface of a chosen transducer, of an oligonucleotide with a specific base sequence called capture probe. The complementary sequence (the analytical target, i.e. a specific sequence of the DNA/RNA of the pathogen) present in the sample is recognized and captured by the probe through the hybridization reaction. The evaluation of the extent of the hybridization allows one to confirm whether the sample contains the complementary sequence of the probe or not. Electrochemical transducers have received considerable attention in connection with the detection of DNA hybridization. Moreover, recently, with the emergence of novel photoelectrochemically active species and new detection schemes, photoelectrochemistry has resulted in substantial progress in its analytical performance for biosensing applications. In this paper, some examples of electrochemical genosensors for multiplexed pathogen detection are shown. Moreover, the preliminary experiments towards the development of a photoelectrochemical genosensor using a TiO2-nanocrystal-modified ITO electrode are discussed.
2015
Inglese
Proceedings of SPIE - The International Society for Optical Engineering
Optics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII
fra
21-set-2015
25-set-2015
9781628418620
SPIE
Voccia, D., Bettazi, F., Palchetti, I., Different strategies for the detection of bioagents using electrochemical and photoelectrochemical genosensors, Abstract de <<Optics and Photonics for Counterterrorism, Crime Fighting, and Defence XI; and Optical Materials and Biomaterials in Security and Defence Systems Technology XII>>, (fra, 21-25 September 2015 ), SPIE, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA 2015:<<PROCEEDINGS OF SPIE, THE INTERNATIONAL SOCIETY FOR OPTICAL ENGINEERING>>,9652 96520T-96520T. 10.1117/12.2194838 [http://hdl.handle.net/10807/155917]
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/10807/155917
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