https://hdl.handle.net/10807/225 Fri, 13 Nov 2026 07:24:02 GMT 2026-11-13T07:24:02Z https://hdl.handle.net/10807/350737 Titolo: Pratiche, funzioni e significati del museo agazziano nella scuola materna novecentesca Autori: Renata Bressanelli Abstract: Il contributo focalizza la sua attenzione sui musei scolastici, ovvero quelle raccolte di oggetti che, dalla seconda metà dell’Ottocento nel quadro dell’affermazione del positivismo e della costituzione dei sistemi scolastici nazionali, registrarono un progressivo sviluppo nelle scuole europee. Lo studio si inserisce nel filone delle ricerche nazionali e internazionali condotte sul patrimonio educativo e sull’insegnamento oggettivo. Nello specifico, esamina il museo ideato dalle sorelle Rosa e Carolina Agazzi in seno all’esperimento di educazione infantile che esse promossero, sotto la guida di Pietro Pasquali, presso l’asilo rurale di Mompiano (Brescia). L'intreccio delle informazioni raccolte da una pluralità di fonti (carte d'archivio, riviste per le educatrici, collezioni didattiche) consente di “fare parlare” questo museo, gettando luce non solo sulle idealità pedagogiche ad esso sottese, ma anche sui bisogni didattici ed educativi di cui si è fatto espressione nella realtà quotidiana degli asili novecenteschi. Thu, 01 Jan 2026 00:00:00 GMT https://hdl.handle.net/10807/350737 2026-01-01T00:00:00Z https://hdl.handle.net/10807/350378 Titolo: ANALYSIS OF LONG-READ GENOME DATA AND POPULATION GENOMICS OF THE SUMMER TRUFFLE (TUBER AESTIVUM) Autori: MILITELLO N.; SOFFRITTI G.; STAGNATI L.; LANUBILE A.; PUGLISI E.; BUSCONI M. Abstract: Truffles are relevant ectomycorrhizal fungi, both for their ecological role and for their economic value. They establish symbiotic association with different host plants, through which they contribute to soil functioning, nutrient cycling, and forest ecosystem stability. In addition, truffle production represents an important resource for local economies, particularly in marginal rural areas. In the Apennines, increasing interest has been directed toward the cultivation of the summer truffle (Tuber aestivum) as a strategy to recover abandoned woodlands and enhance the sustainable use of forest landscapes. Within a broader project aimed at investigating the genetic diversity and evolutionary history of T. aestivum, a long-read genome assembly was obtained (total size of scaffolds 144300040, number of scaffolds 24, GC content 48.5%, longest scaffold 13866245, and percentage of scaffolds > 1M nt 91.7%) with the aim of contributing to a better genomic understanding of this species. The availability of a high-quality genome assembly provides a valuable resource for studying genomic structure and variability in this species. Therefore, core genes, repetitive elements, genome organization, and other relevant genomic features were analysed with the aim of presenting an integrated genomic characterization relevant to population structure, adaptation, and diversification. Thu, 01 Jan 2026 00:00:00 GMT https://hdl.handle.net/10807/350378 2026-01-01T00:00:00Z https://hdl.handle.net/10807/350377 Titolo: LIPID–HORMONE CROSSTALK MEDIATED BY WRKY125 AND LOX4 ENHANCES RESISTANCE TO FUSARIUM VERTICILLIOIDES IN MAIZE Autori: LANUBILE A.; OTTAVIANI L.; LEFEUVRE R.; MONTES E.; GIORNI P.; DALL’ASTA C.; MITHÖFER A.; WIDIEZ T.; MAROCCO A. Abstract: Fusarium verticillioides (Fv) is a major cereal pathogen causing stalk rot and ear rot in maize, negatively affecting crop productivity, and compromising food safety by producing the secondary metabolites fumonisins. Several studies were conducted to identify maize genes associated with host plant resistance to Fv infection and fumonisin accumulation. The maize WRKY transcription factors and the lipoxygenases (LOXs) are well recognized as important players in plant defense against pathogens, and it is known that the host-pathogen lipid crosstalk influences the pathogenesis. In this regard, previous RNA-seq experiments reported the enhanced expression of LOX genes in maize resistant genotypes and GWAS resulted in one SNP significantly associated with WRKY125. The Clustered Regularly Interspaced Short Palindromic Repeat/associated Cas9 (CRISPR/Cas9) editing of WRKY125 and the transgenic overexpression of LOX4 genes were carried out to investigate the possible implication of these two genes in the resistance mechanisms against Fv. Wrky125 edited lines showed a decrease by about 5 and 4 times of Fusarium ear rot severity and fumonisin contamination, respectively, compared to the wild-type genotype. RNA-seq analysis highlighted an enhanced modulation of the jasmonic acid (JA) and abscisic acid (ABA) hormones, redox state, cell wall modification, and secondary metabolism-associated genes after fungal infection in the mutant background in contrast to wildtype. Moreover, the increased expression of JA- and ABA-related genes correlated with a wider accumulation of these two phytohormones. LOX4 overexpression also resulted in enhanced resistance to Fv infection and fumonisin contamination. Transcriptomic and lipidomic analyses revealed that LOX4 mutants up-regulated expression of 9-LOX and JA-related genes, thereby increasing the production of 9-oxylipins and JA-related metabolites under fungal infection. Further studies will be conducted to explore the effects of WRKY125-editing and LOX4- overexpression on other fungi and abiotic stresses. Thu, 01 Jan 2026 00:00:00 GMT https://hdl.handle.net/10807/350377 2026-01-01T00:00:00Z https://hdl.handle.net/10807/350376 Titolo: TRANSCRIPTIONAL REMODELING IN LEAVES AND BERRIES OF VITIS VINIFERA L. CV. SAUVIGNON BLANC GRAPEVINES SUBJECED TO PARTIAL-DOUBLE PRUNING Autori: STAGNATI L.; LANUBILE A.; OTTAVIANI L.; PELUSI F.; PONI S.; BUSCONI M.; GATTI M. Abstract: Canopy management in vineyard is a key operation affecting both shoot and fruit development. Recently, partial double-pruning has been proposed to delay bunch rot epidemics in European vineyards. We investigated how partial double-pruning (DC) influenced gene expression in leaves and berries of Vitis vinifera L. cv. Sauvignon Blanc grapevines as compared to a traditional single cane-pruning (SC). In 2024, trascriptomics and GO gene ontology analysis were applied on leaf and berry samples collected at two developmental stages (leaves: BBCH 69 and BBCH 73; berries: BBCH 73 and BBCH 79). [FP1] Data are supported by a four-year assessment of grapevine responses encompassing vegetative growth, yield components, and fruit composition. Results point out organ-specific and stage-dependent transcriptional changes in response to DC that are mostly evident during developmental phases characterized by active growth and structural reorganization. The strongest changes occurred at BBCH 73[FP2] , including the upregulation of genes related to cell wall modification and response to stress categories. Genes involved in transcription factors and secondary metabolism functional classes were consistently downregulated early on, suggesting a temporary shift in resource allocation. In contrast, leaves collected at BBCH 69 and berries collected at BBCH 79 showed few changes between SC and DC. Integrating molecular data with agronomical assessment revealed that partial double-pruning is effective to significantly reduce vegetative vigor as compared to SC with less than proportional yield losses [FP3] and almost unvaried fruit composition at harvest. The transcriptomic responses, such as cell wall remodeling in leaves, heat and oxidative stress responses in berries at BBCH 73, and downregulation of secondary metabolism, reflect these physiological adjustments associated with temporary changes in microclimate and canopy structure. Overall, grapevines can react to changes in canopy structure by activating specific molecular pathways rather than broader stress responses. From a practical point of view, this translates in the relevance of partial double-pruning to manage grapevine vigor either for too vigorous vineyards or for plots characterized by strong intra-field variability requiring site-specific actions belonging to precision viticulture strategies. Thu, 01 Jan 2026 00:00:00 GMT https://hdl.handle.net/10807/350376 2026-01-01T00:00:00Z